| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeLauder, Trevor S (12)
|
MDMAC |
54.79 |
52.79 |
-2.00 |
| 2 |
Reice, Noah D (12)
|
MADST |
54.67 |
53.85 |
-0.82 |
| 3 |
Karahalis, Alexander J (12)
|
MADST |
54.15 |
54.01 |
-0.14 |
| 4 |
Truong, Michael (12)
|
PVRMSC |
55.34 |
54.55 |
-0.79 |
| 5 |
Bautz, Fletcher H (12)
|
PVRMSC |
56.23 |
55.25 |
-0.98 |
| 6 |
Quach, Harrison T (12)
|
PVRMSC |
56.44 |
55.67 |
-0.77 |
| 7 |
Skelly, Kyle D (12)
|
MADST |
56.73 |
56.10 |
-0.63 |
| 8 |
Truong, Dawson K (12)
|
PVOCCS |
57.82 |
56.31 |
-1.51 |
| 9 |
Jacome, Christopher H (12)
|
PVRMSC |
56.84 |
57.09 |
0.25 |
| 10 |
Rowland, Logan N (12)
|
MDMAC |
56.63 |
58.01 |
1.38 |
| 11 |
Newland, Bennett A (12)
|
PVOCCS |
58.51 |
58.54 |
0.03 |
| 12 |
Texter, Jacob C (12)
|
MADST |
59.86 |
58.71 |
-1.15 |
| 13 |
Brown, Aidan M (12)
|
MDMAC |
58.30 |
59.38 |
1.08 |
| 14 |
Pearson, Boden J (12)
|
PVOCCS |
1:00.08 |
1:00.07 |
-0.01 |
| 15 |
Kolias, Frederick M (12)
|
MDMAC |
1:05.61 |
1:04.63 |
-0.98 |